Mass Spectrometry Lab Rotation A
This course will be a hands-on intensive laboratory rotation. Students will learn how to prepare samples that are "mass spec ready" from a variety of liquid and solid tissue biological samples. Students will learn quality control parameters and will produce high quality samples for use in the HPLC and mass spectrometer.
Prerequisites: Enrollment in CAMS-MS program.
Outcomes: At the end of this course students will be able to: 1) Process liquid and tissue biological samples for analysis by mass spectrometry; 2) Perform gel electrophoresis on prepared samples and excise selected gel bands; 3) Perform in in-gel digestions using standard enzymatic approaches; 4) Perform in-solution digestions using standard enzymatic approaches; 5) Quantify protein samples before and after digestion; and 6) Use column chromatography to generate high purity samples.
Prerequisites: Enrollment in CAMS-MS program.
Outcomes: At the end of this course students will be able to: 1) Process liquid and tissue biological samples for analysis by mass spectrometry; 2) Perform gel electrophoresis on prepared samples and excise selected gel bands; 3) Perform in in-gel digestions using standard enzymatic approaches; 4) Perform in-solution digestions using standard enzymatic approaches; 5) Quantify protein samples before and after digestion; and 6) Use column chromatography to generate high purity samples.