This laboratory is designed to simulate a research experience and to teach basic techniques utilized in a biochemistry laboratory. The course theme involves a comparative investigation of the enzyme glyceraidehyde-3-phosphate dehydrogenase (GAPDH) from various animal sources. All procedures required in lab will be found by the student in the library and proposed to the instructor(s) as a pre-lab exercise. Each two-student team will be working on GAPDH from either an aquatic or land animal source, e.g., trout, tuna, pork beef or chicken.
Prerequisites: CHEM 242 or CHEM 222 or CHEM 226; and (CHEM 260 and CHEM 272) or (CHEM 212 and CHEM 214); and CHEM 370; Restricted to Biochemistry majors.
Outcomes: Students will be able to purify and characterize an enzyme in a research context.
Prerequisites: CHEM 242 or CHEM 222 or CHEM 226; and (CHEM 260 and CHEM 272) or (CHEM 212 and CHEM 214); and CHEM 370; Restricted to Biochemistry majors.
Outcomes: Students will be able to purify and characterize an enzyme in a research context.