This laboratory course is designed to simulate a research project in which molecular biology techniques and biochemistry are integrated. Those techniques are used as important tools to help solve questions in enzyme structure and function. he course theme involves an investigation on the relationship between protein structure and function of the ADP-glucose pyrophosphorylase (ADP-Glc PPase) from Escherichia coli. All procedure required in lab will be found by the student in the library and proposed to the instructor(s) as a pre-lab exercise. Each two-student team will be working on a specific ADP-Glc PPase that has been previously obtained in a recombinant form. Their genes will be provided in a plasmid form.
Outcomes: Students will study the basic concepts of enzyme structure relationships and how to investigate them. The student will learn strategies to produce and test a hypothesis in this area. Additionally, the student will learn how to integrate molecular biology techniques and biochemistry.
Outcomes: Students will study the basic concepts of enzyme structure relationships and how to investigate them. The student will learn strategies to produce and test a hypothesis in this area. Additionally, the student will learn how to integrate molecular biology techniques and biochemistry.